World meals safety calls for sustainable options to the standard feed ingredient wheat. Nutrient-dense diploid potatoes (HQP) characterize candidates because of their enriched phytochemical profiles, together with excessive ranges of vitamin C and lutein. Nonetheless, their dietary utilization is constrained by endogenous anti-nutritional components (ANFs), primarily solanine and trypsin inhibitors. Utilizing a zebrafish mannequin, we display that these ANFs set off intestinal dysbiosis and an oxidative-inflammatory response. Mechanistically, this antagonistic intestine atmosphere suppresses the mTOR pathway, as evidenced by lowered phosphorylation of S6K1 and 4E-BP1, finally resulting in progress despair. To handle this toxicological barrier, we built-in genetic choice (diploid HQP) with thermal processing (steam-cooking). Quantitative evaluation revealed that whereas thermal processing successfully lowered ANF concentrations to physiologically protected ranges, the HQP matrix retained considerably greater residual concentrations of vitamin C, lutein, and zeaxanthin in comparison with standard tetraploid (C88) and wheat-based diets. Mechanistically, protein-level proof confirmed that this processed HQP formulation promoted the overall NRF2 and suppressed NF-κB P65 accumulation. This metabolic shift attenuated irritation, reactivated mTOR signaling, and subsequently enhanced progress efficiency and digestive capability. Our findings set up a mechanistic framework demonstrating how built-in genomic design and processing methods facilitate the purposeful utilization of high-phytochemical crops in sustainable meals techniques.
