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Source link <\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Stalder, J. et al. Tissue-specific DNA cleavages within the globin chromatin area launched by DNAase I. Cell 20, 451\u2013460 (1980). Article\u00a0 Google Scholar\u00a0 Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y. & Greenleaf, W. J. Transposition of native chromatin for quick and delicate epigenomic profiling of open chromatin, DNA-binding proteins and […]<\/p>\n","protected":false},"author":1,"featured_media":419,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png","fifu_image_alt":"","jnews-multi-image_gallery":[],"jnews_single_post":[],"jnews_primary_category":[],"jnews_override_bookmark_settings":[],"jnews_social_meta":[],"jnews_override_counter":[],"footnotes":""},"categories":[10],"tags":[662,665,664,663],"class_list":["post-417","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biotechnology","tag-enzyme","tag-epigenomics","tag-situ","tag-tethering"],"yoast_head":"\nEnzyme tethering for in situ epigenomics - Future News 24<\/title>\n<meta name=\"description\" content=\"Genome-wide mapping of factors that bind, package, replicate and transcribe DNA continues to be challenging despite rapid advances in DNA sequencing technologies. For nearly two decades, chromatin immunoprecipitation with sequencing (ChIP–seq) has dominated epigenomic mapping applications; however, alternative methods for epigenomic mapping have recently begun to replace ChIP–seq. The most popular alternatives use enzymes that modify, cleave or transpose DNA, using freely diffusing enzymes for chromatin accessibility mapping, or tethered ones to specific chromatin proteins or modifications for epigenomic profiling. Unlike ChIP–seq, which solubilizes chromatin before immunoprecipitation, enzyme-tethering epigenomic methods maintain cells or nuclei intact during DNA modification, cleavage or transposition. Although the basic in vivo and in situ enzyme-tethering technologies were introduced more than 20 years ago, they are especially well suited for current epigenomic challenges, including single-cell and spatial applications and both DNA-based and RNA-based time-resolved epigenomic mapping. In this Primer, we discuss DNA modification-based (DamID), cleavage-based (ChIC and CUT&amp;RUN) and transposase-based (CUT&amp;Tag and RT&amp;Tag) tethered-enzyme profiling methods, and describe protocols for data processing, quality control and data analysis used by the epigenomics community. We anticipate that advances in enzyme engineering and automation will make enzyme-tethering methods increasingly attractive for both large and small epigenomic profiling projects. Enzyme-tethering epigenomic methods modify DNA while cells and nuclei remain intact, making them particularly suitable for single-cell and spatial applications. In this Primer, Kami Ahmad et al. discuss the various approaches for enzyme-tethering analysis and major applications in biological research.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Enzyme tethering for in situ epigenomics - Future News 24\" \/>\n<meta property=\"og:description\" content=\"Genome-wide mapping of factors that bind, package, replicate and transcribe DNA continues to be challenging despite rapid advances in DNA sequencing technologies. For nearly two decades, chromatin immunoprecipitation with sequencing (ChIP–seq) has dominated epigenomic mapping applications; however, alternative methods for epigenomic mapping have recently begun to replace ChIP–seq. The most popular alternatives use enzymes that modify, cleave or transpose DNA, using freely diffusing enzymes for chromatin accessibility mapping, or tethered ones to specific chromatin proteins or modifications for epigenomic profiling. Unlike ChIP–seq, which solubilizes chromatin before immunoprecipitation, enzyme-tethering epigenomic methods maintain cells or nuclei intact during DNA modification, cleavage or transposition. Although the basic in vivo and in situ enzyme-tethering technologies were introduced more than 20 years ago, they are especially well suited for current epigenomic challenges, including single-cell and spatial applications and both DNA-based and RNA-based time-resolved epigenomic mapping. In this Primer, we discuss DNA modification-based (DamID), cleavage-based (ChIC and CUT&amp;RUN) and transposase-based (CUT&amp;Tag and RT&amp;Tag) tethered-enzyme profiling methods, and describe protocols for data processing, quality control and data analysis used by the epigenomics community. We anticipate that advances in enzyme engineering and automation will make enzyme-tethering methods increasingly attractive for both large and small epigenomic profiling projects. Enzyme-tethering epigenomic methods modify DNA while cells and nuclei remain intact, making them particularly suitable for single-cell and spatial applications. In this Primer, Kami Ahmad et al. discuss the various approaches for enzyme-tethering analysis and major applications in biological research.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/\" \/>\n<meta property=\"og:site_name\" content=\"Future News 24\" \/>\n<meta property=\"article:published_time\" content=\"2026-06-04T00:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-06-04T18:44:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png\" \/>\n<meta name=\"author\" content=\"Future News 24\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Future News 24\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"27 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/\"},\"author\":{\"name\":\"Future News 24\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#\\\/schema\\\/person\\\/cecad1bde21cfc357cf70128144d6c83\"},\"headline\":\"Enzyme tethering for in situ epigenomics\",\"datePublished\":\"2026-06-04T00:00:00+00:00\",\"dateModified\":\"2026-06-04T18:44:32+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/\"},\"wordCount\":5478,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/media.springernature.com\\\/m685\\\/springer-static\\\/image\\\/art%3A10.1038%2Fs43586-026-00491-6\\\/MediaObjects\\\/43586_2026_491_Fig1_HTML.png\",\"keywords\":[\"Enzyme\",\"epigenomics\",\"situ\",\"tethering\"],\"articleSection\":[\"BioTechnology\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/\",\"url\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/\",\"name\":\"Enzyme tethering for in situ epigenomics - Future News 24\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/06\\\/04\\\/s43586-026-00491-6\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/media.springernature.com\\\/m685\\\/springer-static\\\/image\\\/art%3A10.1038%2Fs43586-026-00491-6\\\/MediaObjects\\\/43586_2026_491_Fig1_HTML.png\",\"datePublished\":\"2026-06-04T00:00:00+00:00\",\"dateModified\":\"2026-06-04T18:44:32+00:00\",\"description\":\"Genome-wide mapping of factors that bind, package, replicate and transcribe DNA continues to be challenging despite rapid advances in DNA sequencing technologies. 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In this Primer, Kami Ahmad et al. discuss the various approaches for enzyme-tethering analysis and major applications in biological research.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/","og_locale":"en_US","og_type":"article","og_title":"Enzyme tethering for in situ epigenomics - Future News 24","og_description":"Genome-wide mapping of factors that bind, package, replicate and transcribe DNA continues to be challenging despite rapid advances in DNA sequencing technologies. For nearly two decades, chromatin immunoprecipitation with sequencing (ChIP–seq) has dominated epigenomic mapping applications; however, alternative methods for epigenomic mapping have recently begun to replace ChIP–seq. The most popular alternatives use enzymes that modify, cleave or transpose DNA, using freely diffusing enzymes for chromatin accessibility mapping, or tethered ones to specific chromatin proteins or modifications for epigenomic profiling. Unlike ChIP–seq, which solubilizes chromatin before immunoprecipitation, enzyme-tethering epigenomic methods maintain cells or nuclei intact during DNA modification, cleavage or transposition. Although the basic in vivo and in situ enzyme-tethering technologies were introduced more than 20 years ago, they are especially well suited for current epigenomic challenges, including single-cell and spatial applications and both DNA-based and RNA-based time-resolved epigenomic mapping. In this Primer, we discuss DNA modification-based (DamID), cleavage-based (ChIC and CUT&amp;RUN) and transposase-based (CUT&amp;Tag and RT&amp;Tag) tethered-enzyme profiling methods, and describe protocols for data processing, quality control and data analysis used by the epigenomics community. We anticipate that advances in enzyme engineering and automation will make enzyme-tethering methods increasingly attractive for both large and small epigenomic profiling projects. Enzyme-tethering epigenomic methods modify DNA while cells and nuclei remain intact, making them particularly suitable for single-cell and spatial applications. In this Primer, Kami Ahmad et al. discuss the various approaches for enzyme-tethering analysis and major applications in biological research.","og_url":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/","og_site_name":"Future News 24","article_published_time":"2026-06-04T00:00:00+00:00","article_modified_time":"2026-06-04T18:44:32+00:00","og_image":[{"url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png","type":"","width":"","height":""}],"author":"Future News 24","twitter_card":"summary_large_image","twitter_image":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png","twitter_misc":{"Written by":"Future News 24","Est. reading time":"27 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/#article","isPartOf":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/"},"author":{"name":"Future News 24","@id":"https:\/\/futurenews24.com\/#\/schema\/person\/cecad1bde21cfc357cf70128144d6c83"},"headline":"Enzyme tethering for in situ epigenomics","datePublished":"2026-06-04T00:00:00+00:00","dateModified":"2026-06-04T18:44:32+00:00","mainEntityOfPage":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/"},"wordCount":5478,"commentCount":0,"publisher":{"@id":"https:\/\/futurenews24.com\/#organization"},"image":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/#primaryimage"},"thumbnailUrl":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png","keywords":["Enzyme","epigenomics","situ","tethering"],"articleSection":["BioTechnology"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/","url":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/","name":"Enzyme tethering for in situ epigenomics - Future News 24","isPartOf":{"@id":"https:\/\/futurenews24.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/#primaryimage"},"image":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/06\/04\/s43586-026-00491-6\/#primaryimage"},"thumbnailUrl":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs43586-026-00491-6\/MediaObjects\/43586_2026_491_Fig1_HTML.png","datePublished":"2026-06-04T00:00:00+00:00","dateModified":"2026-06-04T18:44:32+00:00","description":"Genome-wide mapping of factors that bind, package, replicate and transcribe DNA continues to be challenging despite rapid advances in DNA sequencing technologies. For nearly two decades, chromatin immunoprecipitation with sequencing (ChIP–seq) has dominated epigenomic mapping applications; however, alternative methods for epigenomic mapping have recently begun to replace ChIP–seq. The most popular alternatives use enzymes that modify, cleave or transpose DNA, using freely diffusing enzymes for chromatin accessibility mapping, or tethered ones to specific chromatin proteins or modifications for epigenomic profiling. Unlike ChIP–seq, which solubilizes chromatin before immunoprecipitation, enzyme-tethering epigenomic methods maintain cells or nuclei intact during DNA modification, cleavage or transposition. Although the basic in vivo and in situ enzyme-tethering technologies were introduced more than 20 years ago, they are especially well suited for current epigenomic challenges, including single-cell and spatial applications and both DNA-based and RNA-based time-resolved epigenomic mapping. In this Primer, we discuss DNA modification-based (DamID), cleavage-based (ChIC and CUT&amp;RUN) and transposase-based (CUT&amp;Tag and RT&amp;Tag) tethered-enzyme profiling methods, and describe protocols for data processing, quality control and data analysis used by the epigenomics community. We anticipate that advances in enzyme engineering and automation will make enzyme-tethering methods increasingly attractive for both large and small epigenomic profiling projects. Enzyme-tethering epigenomic methods modify DNA while cells and nuclei remain intact, making them particularly suitable for single-cell and spatial applications. 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