\n
Wegierak, D. et al. Nanobubble distinction enhanced ultrasound imaging: a evaluate. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 16, e2007 (2024).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Ferrara, Okay. W., Borden, M. A. & Zhang, H. Lipid-shelled autos: engineering for ultrasound molecular imaging and drug supply. Acc. Chem. Res. 42, 881\u2013892 (2009).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Krupka, T. M. et al. Formulation and characterization of echogenic lipid\u2212pluronic nanobubbles. Mol. Pharm. 7, 49\u201359 (2010).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Paefgen, V., Doleschel, D. & Kiessling, F. Evolution of distinction brokers for ultrasound imaging and ultrasound-mediated drug supply. Entrance. Pharmacol. 6, 197 (2015).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Feinstein, S. B. et al. Security and efficacy of a brand new transpulmonary ultrasound distinction agent: preliminary multicenter scientific outcomes. J. Am. Coll. Cardiol. 16, 316\u2013324 (1990).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Exner, A. A. & Kolios, M. C. Bursting microbubbles: how nanobubble distinction brokers can allow the way forward for medical ultrasound molecular imaging and image-guided remedy. Curr. Opin. Colloid Interface Sci. 54, 101463 (2021).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wu, H. et al. Time-intensity-curve evaluation and tumor extravasation of nanobubble ultrasound distinction brokers. Ultrasound Med. Biol. 45, 2502\u20132514 (2019).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Pellow, C., O\u2019Reilly, M. A., Hynynen, Okay., Zheng, G. & Goertz, D. E. Simultaneous intravital optical and acoustic monitoring of ultrasound-triggered nanobubble technology and extravasation. Nano Lett. 20, 4512\u20134519 (2020).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Ramirez, D. G. et al. Distinction-enhanced ultrasound with sub-micron sized distinction brokers detects insulitis in mouse fashions of type1 diabetes. Nat. Commun. 11 (2020).<\/p>\n
de Leon, A. et al. Distinction enhanced ultrasound imaging by nature-inspired ultrastable echogenic nanobubbles. Nanoscale 11, 15647\u201315658 (2019).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cooley, M. B. et al. Characterization of the interplay of nanobubble ultrasound distinction brokers with human blood parts. Bioact. Mater. 19, 642\u2013652 (2023).<\/p>\n
\n Google Scholar\u00a0\n <\/p>\n
Chen, C. et al. Pharmacokinetic modeling of the second-wave phenomenon in nanobubble-based contrast-enhanced ultrasound. IEEE Trans. Biomed. Eng. https:\/\/doi.org\/10.1109\/TBME.2022.3184266 (2022).<\/p>\n
Chen, C. et al. Pharmacokinetic modeling of PSMA-targeted nanobubbles for quantification of extravasation and binding in mice fashions of prostate most cancers. Med. Phys. 49, 6547\u20136559 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Hysi, E. et al. Photoacoustic imaging biomarkers for monitoring biophysical modifications throughout nanobubble-mediated radiation therapy. Photoacoustics 20, 100201 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Chen, C. et al. The distinctive second wave phenomenon in distinction enhanced ultrasound imaging with nanobubbles. Sci. Rep. 12, 13619 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Moore, M. J. et al. The dance of the nanobubbles: detecting acoustic backscatter from sub-micron bubbles utilizing ultra-high frequency acoustic microscopy. Nanoscale 12, 21420\u201321428 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Bismuth, M. et al. Low frequency nanobubble-enhanced ultrasound mechanotherapy for noninvasive most cancers surgical procedure. Nanoscale 14, 13614\u201313627 (2022).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cooley, M. B. et al. Actual-time imaging of nanobubble ultrasound distinction agent circulation, extravasation, and diffusion by way of an extracellular matrix utilizing a microfluidic mannequin. Lab Chip 23, 3453\u20133466 (2023).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Pellow, C., Abenojar, E. C., Exner, A. A., Zheng, G. & Goertz, D. E. Concurrent visible and acoustic monitoring of passive and energetic supply of nanobubbles to tumors. Theranostics 10, 11690\u201311706 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Perera, R. H. et al. Intracellular vesicle entrapment of nanobubble ultrasound distinction brokers focused to PSMA promotes extended enhancement and stability in vivo and in vitro. Nanotheranostics 6, 270\u2013285 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wegierak, D. et al. Results of shell-integrated Sudan Black dye on the acoustic exercise and ultrasound imaging properties of lipid-shelled nanoscale ultrasound distinction brokers. J. Biomed. Choose. 27, 016501 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wegierak, D. et al. Decorrelation time mapping as an evaluation device for nanobubble-based distinction enhanced ultrasound imaging. IEEE Trans. Med. Imaging 46, 2370\u20132380 (2024).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Johansen, M. L. et al. Ultrasound-based molecular imaging of tumors with PTPmu biomarker-targeted nanobubble distinction brokers. Int. J. Mol. Sci. 22, 1983 (2021).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Foudas, A. W. et al. Fundamentals and functions of nanobubbles: a evaluate. Chem. Eng. Res. Des. 189, 64\u201386 (2023).<\/p>\n
Wang, Y. et al. Growth of a novel castration-resistant orthotopic prostate most cancers mannequin in New Zealand white rabbit. Prostate 82, 695\u2013705 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Perera, R. H. et al. Enhancing efficiency of nanoscale ultrasound distinction brokers utilizing N,N-diethylacrylamide stabilization. Nanomedicine 13, 59\u201367 (2017).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yin, T. et al. Nanobubbles for enhanced ultrasound imaging of tumors. Int. J. Nanomed. 7, 895\u2013904 (2012).<\/p>\n
\n Google Scholar\u00a0\n <\/p>\n
Liu, J. et al. Ultrasound molecular imaging of acute cardiac transplantation rejection utilizing nanobubbles focused to T lymphocytes. Biomaterials 162, 200\u2013207 (2018).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
de Leon, A. et al. Ultrasound distinction brokers and supply techniques in most cancers detection and remedy. Adv. Most cancers Res. 139, 57\u201384 (2018).<\/p>\n
Perera, R. H. et al. Actual time ultrasound molecular imaging of prostate most cancers with PSMA-targeted nanobubbles. Nanomedicine 28, 102213 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wang, Y. et al. Molecular imaging of orthotopic prostate most cancers with nanobubble ultrasound distinction brokers focused to PSMA. Sci. Rep. 11, 4726 (2021).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Abenojar, E. C. et al. Impact of bubble focus on the in vitro and in vivo efficiency of extremely steady lipid shell-stabilized micro- and nanoscale ultrasound distinction brokers. Langmuir 35, 10192\u201310202 (2019).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yu, Z. et al. Anti-G250 nanobody-functionalized nanobubbles focusing on renal cell carcinoma cells for ultrasound molecular imaging. Nanotechnology 31, 205101 (2020).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Gao, Y. et al. Ultrasound molecular imaging of ovarian most cancers with CA-125 focused nanobubble distinction brokers. Nanomedicine 13, 2159\u20132168 (2017).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Jiang, Q. et al. Manufacturing and characterization of a novel long-acting herceptin-targeted nanobubble distinction agent particular for Her-2-positive breast cancers. Breast Most cancers 23, 445\u2013455 (2016).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Li, J. et al. Neuropeptide Y Y1 receptor-mediated biodegradable photoluminescent nanobubbles as ultrasound distinction brokers for focused breast most cancers imaging. Biomaterials 116, 106\u2013117 (2017).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Zhu, L. et al. Development of ultrasonic nanobubbles carrying CAIX polypeptides to focus on carcinoma cells derived from varied organs. J. Nanobiotechnol. 15, 63 (2017).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Fan, X. et al. Experimental investigation of the penetration of ultrasound nanobubbles in a gastric most cancers xenograft. Nanotechnology 24, 325102 (2013).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yang, H. et al. Preparation of multifunctional nanobubbles and their software in bimodal imaging and focused mixture remedy of early pancreatic most cancers. Sci. Rep. 11, 6254 (2021).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cooley, M. B. et al. Assessing therapeutic nanoparticle accumulation in tumors utilizing nanobubble-based contrast-enhanced ultrasound imaging. ACS Nano 18, 33181\u201333196 (2024).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Xie, F. et al. Analysis of liver ischemia\u2013reperfusion harm in rabbits utilizing a nanoscale ultrasound distinction agent focusing on ICAM-1. PLoS ONE 11, e0153805 (2016).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Argenziano, M. et al. Vancomycin-loaded nanobubbles: a brand new platform for managed antibiotic supply in opposition to methicillin-resistant Staphylococcus aureus infections. Int. J. Pharm. 523, 176\u2013188 (2017).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cavalli, R. et al. Preparation and in vitro characterization of chitosan nanobubbles as theranostic brokers. Colloids Surf. B 129, 39\u201346 (2015).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Shen, J. et al. Resveratrol supply by ultrasound-mediated nanobubbles focusing on nucleus pulposus cells. Nanomedicine 13, 1433\u20131446 (2018).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Nittayacharn, P., de Leon, A., Abenojar, E. C. & Exner, A. A. The impact of lipid solubilization on the efficiency of doxorubicin-loaded nanobubbles. In 2018 IEEE Worldwide Ultrasonics Symposium 8579716 (IEEE, 2018).<\/p>\n
Nittayacharn, P., Abenojar, E., De Leon, A., Wegierak, D. & Exner, A. A. Growing doxorubicin loading in lipid-shelled perfluoropropane nanobubbles through a easy deprotonation technique. Entrance. Pharmacol. 11, 644 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Lin, W. et al. Cell-penetrating peptide-doxorubicin conjugate loaded NGR-modified nanobubbles for ultrasound triggered drug supply. J. Drug Goal. 24, 134\u2013146 (2016).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Zhang, X. et al. Methotrexate-loaded PLGA nanobubbles for ultrasound imaging and synergistic focused remedy of residual tumor throughout HIFU ablation. Biomaterials 35, 5148\u20135161 (2014).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wang, J.-P. et al. Paclitaxel-loaded nanobubble focused to pro-gastrin-releasing peptide inhibits the expansion of small cell lung most cancers. Most cancers Manag. Res. 11, 6637\u20136649 (2019).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Kida, H. et al. Environment friendly mRNA supply with lyophilized human serum albumin-based nanobubbles. Nanomaterials 13, 1283 (2023).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Su, C. et al. Ultrasound-sensitive siRNA-loaded nanobubbles fabrication and antagonism in drug resistance for NSCLC. Drug Deliv. 29, 99\u2013110 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Kida, H. et al. Nanobubble mediated gene supply along with a hand-held ultrasound scanner. Entrance. Pharmacol. 11, 363 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Watanabe, Y. et al. Supply of Na\/I symporter gene into skeletal muscle utilizing nanobubbles and ultrasound: visualization of gene expression by PET. J. Nucl. Med. 51, 951\u2013958 (2010).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yin, T. et al. Tumor-penetrating codelivery of siRNA and paclitaxel with ultrasound-responsive nanobubbles hetero-assembled from polymeric micelles and liposomes. Biomaterials 35, 5932\u20135943 (2014).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Gao, J. et al. Ultrasound-assisted C3F8-filled PLGA nanobubbles for enhanced FGF21 supply and improved prophylactic therapy of diabetic cardiomyopathy. Acta Biomater. 130, 395\u2013408 (2021).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Mi, X. et al. Asparagine endopeptidase-targeted ultrasound-responsive nanobubbles alleviate tau cleavage and amyloid-\u03b2 deposition in an Alzheimer\u2019s illness mannequin. Acta Biomater. 141, 388\u2013397 (2022).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yan, Y. et al. Mind supply of curcumin by way of low-intensity ultrasound-induced blood\u2013mind barrier opening through lipid\u2013PLGA nanobubbles. Int. J. Nanomed. 16, 7433\u20137447 (2021).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cheng, B., Bing, C. & Chopra, R. The impact of transcranial targeted ultrasound goal location on the acoustic suggestions management efficiency throughout blood\u2013mind barrier opening with nanobubbles. Sci. Rep. 9, 20020 (2019).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cheng, B. et al. Affect of nanobubble focus on blood\u2013mind barrier opening utilizing targeted ultrasound beneath real-time acoustic suggestions management. Ultrasound Med. Biol. 45, 2174\u20132187 (2019).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Nittayacharn, P. et al. Environment friendly ultrasound-mediated drug supply to orthotopic liver tumors\u2014direct comparability of doxorubicin-loaded nanobubbles and microbubbles. J. Management. Launch 367, 135\u2013147 (2024).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wang, Y. et al. Focused Sudan Black nanobubbles as photoacoustic distinction brokers for breast most cancers imaging. In Photons Plus Ultrasound: Imaging and Sensing 2019 (eds Oraevsky, A. A. & Wang, L. V.) 85 (SPIE, 2019); https:\/\/doi.org\/10.1117\/12.2510876.<\/p>\n
Nittayacharn, P. et al. Iridium(III) complex-loaded perfluoropropane nanobubbles for enhanced sonodynamic remedy. Bioconjug. Chem. 33, 1057\u20131068 (2022).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yi, X., Abenojar, E. C., Zhu, J., Zheng, Y. & Exner, A. A. In vitro preparation and characterization of magnetic nanobubbles. In 2019 IEEE Worldwide Ultrasonics Symposium 392\u2013395 (IEEE, 2019); https:\/\/doi.org\/10.1109\/ULTSYM.2019.8925956.<\/p>\n
Jin, Z. et al. Tumor focused multifunctional magnetic nanobubbles for MR\/US twin imaging and targeted ultrasound triggered drug supply. Entrance. Bioeng. Biotechnol. 8, 586874 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Zhu, Y. et al. Magnetic polymeric nanobubbles with optimized core dimension for MRI\/ultrasound bimodal molecular imaging of prostate most cancers. Nanomedicine 15, 2901\u20132916 (2020).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Sojahrood, A. J. et al. Towards exactly controllable acoustic response of shell-stabilized nanobubbles: excessive yield and slim dispersity. ACS Nano 15, 4901\u20134915 (2021).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Sojahrood, A. J. et al. Nonlinear dynamics of acoustic bubbles excited by their pressure-dependent subharmonic resonance frequency: affect of the stress amplitude, frequency, encapsulation and a number of bubble interactions on oversaturation and enhancement of the subharmonic sign. Nonlinear Dyn. 103, 429\u2013466 (2021).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Hernandez, C. et al. Sink or float? Characterization of shell-stabilized bulk nanobubbles utilizing a resonant mass measurement approach. Nanoscale 11, 851\u2013855 (2019).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Perera, R. H. et al. Nanobubble ultrasound distinction brokers for enhanced supply of thermal sensitizer to tumors present process radiofrequency ablation. Pharm. Res. 31, 1407\u20131417 (2014).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Hansen, H. H. W. B. et al. Nanobubble applied sciences: functions in remedy from molecular to mobile degree. Biotechnol. Adv. 63, 108091 (2023).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Upadhyay, A. & Dalvi, S. V. microbubble formulations: synthesis, stability, modeling and biomedical functions. Ultrasound Med. Biol. 45, 301\u2013343 (2019).<\/p>\n
Qin, S., Caskey, C. F. & Ferrara, Okay. W. Ultrasound distinction microbubbles in imaging and remedy: bodily rules and engineering. Phys. Med. Biol. 54, R27\u2013R57 (2009).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Batchelor, D. V. B. et al. Nanobubbles for therapeutic supply: manufacturing, stability and present prospects. Curr. Opi. Colloid Interface Sci. 54, 101456 (2021).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Doinikov, A. A., Haac, J. F. & Dayton, P. A. Resonance frequencies of lipid-shelled microbubbles within the regime of nonlinear oscillations. Ultrasonics 49, 263\u2013268 (2009).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Krasovitski, B. & Kimmel, E. Stability of an encapsulated bubble shell. Ultrasonics 44, 216\u2013220 (2006).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Borden, M. A. et al. Affect of lipid shell physicochemical properties on ultrasound-induced microbubble destruction. IEEE Trans. Ultrason. Ferroelectr. Freq. Management 52, 1992\u20132002 (2005).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Dicker, S. et al. Affect of shell composition on the resonance frequency of microbubble distinction brokers. Ultrasound Med. Biol. 39, 1292\u20131302 (2013).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
van Rooij, T. et al. Non-linear response and viscoelastic properties of lipid-coated microbubbles: DSPC versus DPPC. Ultrasound Med. Biol. 41, 1432\u20131445 (2015).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Garg, S., Thomas, A. A. & Borden, M. A. The impact of lipid monolayer in-plane rigidity on in vivo microbubble circulation persistence. Biomaterials 34, 6862\u20136870 (2013).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Batchelor, D. V. B. et al. The affect of nanobubble dimension and stability on ultrasound enhanced drug supply. Langmuir 38, 13943\u201313954 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Park, B. et al. Stability of engineered micro or nanobubbles for biomedical functions. Pharmaceutics 12, 1089 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Perera, R. et al. Nanobubble extravasation in prostate tumors imaged with ultrasound: function of energetic versus passive focusing on. In 2018 IEEE Worldwide Ultrasonics Symposium 8580221 (IEEE, 2018).<\/p>\n
Bosca, F., Bielecki, P. A., Exner, A. A. & Barge, A. Porphyrin-loaded pluronic nanobubbles: a brand new US-activated agent for future theranostic functions. Bioconjug. Chem. 29, 234\u2013240 (2018).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Nieves, L. M. et al. Impact of the surfactant pluronic on the soundness of lipid-stabilized perfluorocarbon nanobubbles. In 2017 IEEE Worldwide Ultrasonics Symposium 8091542 (IEEE, 2017); https:\/\/doi.org\/10.1109\/ULTSYM.2017.8091542.<\/p>\n
Wu, H. et al. Acoustic characterization and pharmacokinetic analyses of latest nanobubble ultrasound distinction brokers. Ultrasound Med. Biol. 39, 2137\u20132146 (2013).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Counil, C., Abenojar, E., Perera, R. & Exner, A. A. Extrusion: a brand new technique for fast formulation of high-yield, monodisperse nanobubbles. Small 18, e2200810 (2022).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yu, Z. et al. G250 antigen-targeting drug-loaded nanobubbles mixed with ultrasound focused nanobubble destruction: a possible novel therapy for renal cell carcinoma. Int. J. Nanomed. 15, 81\u201395 (2020).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yang, H. et al. Nanobubble\u2013Affibody: novel ultrasound distinction brokers for focused molecular ultrasound imaging of tumor. Biomaterials 37, 279\u2013288 (2015).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Peng, Y. et al. Preparation of nanobubbles modified with a small-molecule CXCR4 antagonist for focused drug supply to tumors and enhanced ultrasound molecular imaging. Int. J. Nanomed. 14, 9139\u20139157 (2019).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Abenojar, E. C. et al. Theoretical and experimental gasoline quantity quantification of micro-and nanobubble ultrasound distinction brokers. Pharmaceutics 12, 208 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cooley, M. B., Wegierak, D. & Exner, A. A. Utilizing imaging modalities to foretell nanoparticle distribution and therapy efficacy in strong tumors: the rising function of ultrasound. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 16, e1957 (2024).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Wang, S., Samiotaki, G., Olumolade, O., Feshitan, J. A. & Konofagou, E. E. Microbubble kind and distribution dependence of targeted ultrasound-induced blood\u2013mind barrier opening. Ultrasound Med. Biol. 40, 130\u2013137 (2014).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Bernard, L. et al. ACT 2021 digital annual assembly poster abstracts. Int. J. Toxicol 41, 48\u201377 (2022).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Perera, R. H. et al. Ultrasound-mediated drug-free theranostics for therapy of prostate most cancers. Bioact. Mater. 35, 45\u201355 (2024).<\/p>\n
PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Batchelor, D. V. B. et al. Nested nanobubbles for ultrasound-triggered drug launch. ACS Appl. Mater. Interfaces https:\/\/doi.org\/10.1021\/acsami.0c07022 (2020).<\/p>\n
Khan, M. S. et al. Engineering oxygen nanobubbles for the efficient reversal of hypoxia. Artif. Cells Nanomed. Biotechnol. 46, 318\u2013327 (2018).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Ye, L. et al. Oxygen-loaded lipid nanobubbles for biofilm eradication by mixed trimodal therapy of oxygen, silver, and photodynamic remedy. ACS Appl. Nano Mater. 6, 11715\u201311724 (2023).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Ho, Y.-J. et al. Oxygen-loaded microbubble-mediated sonoperfusion and oxygenation for neuroprotection after ischemic stroke reperfusion. Biomater. Res. 27, 65 (2023).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Cooley, M. B. et al. The impact of nanobubble ultrasound distinction agent shell stiffness and temperature on stability and interactions with purple blood cells. In 2023 IEEE Worldwide Ultrasonics Symposium 10307624 (IEEE, 2023); https:\/\/doi.org\/10.1109\/IUS51837.2023.10307624.<\/p>\n
Reusser, T. D. et al. Phospholipid oxygen microbubbles for image-guided remedy. Nanotheranostics 4, 83\u201390 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Pulsipher, Okay. W., Hammer, D. A., Lee, D. & Sehgal, C. M. Engineering theranostic microbubbles utilizing microfluidics for ultrasound imaging and remedy: a evaluate. Ultrasound Med. Biol. 44, 2441\u20132460 (2018).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Feshitan, J. A., Chen, C. C., Kwan, J. J. & Borden, M. A. Microbubble dimension isolation by differential centrifugation. J. Colloid Interface Sci. 329, 316\u2013324 (2009).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Chen, L. E., Nittayacharn, P. & Exner, A. A. Progress and potential of nanobubbles for ultrasound-mediated drug supply. Professional Opin. Drug Deliv. 22, 1007\u20131030 (2025).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Yuan, H. et al. Twin-targeted microbubbles particular to integrin \u03b1V\u03b23 and vascular endothelial development issue receptor 2 for ultrasonography analysis of tumor angiogenesis. Ultrasound Med. Biol. 44, 1460\u20131467 (2018).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Endo-Takahashi, Y. & Negishi, Y. Microbubbles and nanobubbles with ultrasound for systemic gene supply. Pharmaceutics 12, 964 (2020).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Nittayacharn, P., Dai, Okay., De Leon, A., Therdrattanawong, C. & Exner, A. A. The impact of freeze\/thawing on the bodily properties and acoustic efficiency of perfluoropropane nanobubble suspensions. In 2019 IEEE Worldwide Ultrasonics Symposium 2279\u20132282 (IEEE, 2019); https:\/\/doi.org\/10.1109\/ULTSYM.2019.8926224.<\/p>\n
Hernandez, C., Gulati, S., Fioravanti, G., Stewart, P. L. & Exner, A. A. Cryo-EM visualization of lipid and polymer-stabilized perfluorocarbon gasoline nanobubbles\u2014a step in the direction of nanobubble mediated drug supply. Sci. Rep. 7, 13517 (2017).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Mulvana, H. et al. Characterization of distinction agent microbubbles for ultrasound imaging and remedy analysis. IEEE Trans. Ultrason. Ferroelectr. Freq. Management 64, 232\u2013251 (2017).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Satinover, S. J., Dove, J. D. & Borden, M. A. Single-particle optical sizing of microbubbles. Ultrasound Med Biol 40, 138\u2013147 (2014).<\/p>\n
Article\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n
Sennoga, C. A. et al. Analysis of strategies for sizing and counting of ultrasound distinction brokers. Ultrasound Med. Biol. 38, 834\u2013845 (2012).<\/p>\n
Article\u00a0
\n PubMed Central\u00a0<\/p>\n
Google Scholar\u00a0\n <\/p>\n<\/div>\n
\n
Source link <\/a><\/p>\n","protected":false},"excerpt":{"rendered":"Wegierak, D. et al. Nanobubble distinction enhanced ultrasound imaging: a evaluate. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 16, e2007 (2024). Article\u00a0 PubMed Central\u00a0 Google Scholar\u00a0 Ferrara, Okay. W., Borden, M. A. & Zhang, H. Lipid-shelled autos: engineering for ultrasound molecular imaging and drug supply. Acc. Chem. Res. 42, 881\u2013892 (2009). Article\u00a0 PubMed Central\u00a0 Google Scholar\u00a0 Krupka, […]<\/p>\n","protected":false},"author":1,"featured_media":2740,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png","fifu_image_alt":"","jnews-multi-image_gallery":[],"jnews_single_post":[],"jnews_primary_category":[],"jnews_override_bookmark_settings":[],"jnews_social_meta":[],"jnews_override_counter":[],"footnotes":""},"categories":[10],"tags":[3216,210,1241,3215,3217,3218,3072],"class_list":["post-2738","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biotechnology","tag-activation","tag-agents","tag-contrast","tag-formulation","tag-lipidshelled","tag-nanobubble","tag-ultrasound"],"yoast_head":"\n
Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers - Future News 24<\/title>\n<meta name=\"description\" content=\"Ultrasound is the second most common clinical imaging modality. Ultrasound image quality often suffers from poor contrast resolution and thus can greatly benefit from appropriate contrast agents. While micron-sized gas core particles (microbubbles) are clinically utilized in this space for applications such as echocardiography, a smaller agent could be more broadly applicable to enhance the sensitivity and specificity of disease detection. To this end, lipid-shelled nanobubbles have recently emerged as robust ultrasound contrast agents for both diagnostic and therapeutic purposes. They have been used in preclinical applications ranging from oncology to endocrinology and are notably relevant in diseases that involve pathological vasculature. Owing to their shell composition, nanobubbles are versatile and can be functionalized with fluorophores, targeting agents and therapeutic moieties. Here we provide the steps needed to formulate plain, fluorophore-conjugated, ligand-conjugated, hydrophilic dye-integrated and drug-loaded nanobubbles. The typical formulation for lipid-shelled nanobubbles takes place via self-assembly driven by mechanical agitation, followed by differential centrifugation. The process is deceptively simple, yet there are many nuances in the formulation process that must be followed to produce consistently successful nanobubble batches without contamination from microbubbles. Even minor deviation from the standard protocol can substantially affect nanobubble yield, stability, acoustic performance and batch-to-batch reproducibility. This procedure also details common pitfalls and their potential consequences for bubble quality and performance. The procedure requires 3 h to complete the formulation and activation of the plain nanobubbles by users with basic laboratory expertise. A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers - Future News 24\" \/>\n<meta property=\"og:description\" content=\"Ultrasound is the second most common clinical imaging modality. Ultrasound image quality often suffers from poor contrast resolution and thus can greatly benefit from appropriate contrast agents. While micron-sized gas core particles (microbubbles) are clinically utilized in this space for applications such as echocardiography, a smaller agent could be more broadly applicable to enhance the sensitivity and specificity of disease detection. To this end, lipid-shelled nanobubbles have recently emerged as robust ultrasound contrast agents for both diagnostic and therapeutic purposes. They have been used in preclinical applications ranging from oncology to endocrinology and are notably relevant in diseases that involve pathological vasculature. Owing to their shell composition, nanobubbles are versatile and can be functionalized with fluorophores, targeting agents and therapeutic moieties. Here we provide the steps needed to formulate plain, fluorophore-conjugated, ligand-conjugated, hydrophilic dye-integrated and drug-loaded nanobubbles. The typical formulation for lipid-shelled nanobubbles takes place via self-assembly driven by mechanical agitation, followed by differential centrifugation. The process is deceptively simple, yet there are many nuances in the formulation process that must be followed to produce consistently successful nanobubble batches without contamination from microbubbles. Even minor deviation from the standard protocol can substantially affect nanobubble yield, stability, acoustic performance and batch-to-batch reproducibility. This procedure also details common pitfalls and their potential consequences for bubble quality and performance. The procedure requires 3 h to complete the formulation and activation of the plain nanobubbles by users with basic laboratory expertise. A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/\" \/>\n<meta property=\"og:site_name\" content=\"Future News 24\" \/>\n<meta property=\"article:published_time\" content=\"2026-07-23T00:00:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-07-23T12:59:07+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png\" \/>\n<meta name=\"author\" content=\"Future News 24\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:image\" content=\"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Future News 24\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"14 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/\"},\"author\":{\"name\":\"Future News 24\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#\\\/schema\\\/person\\\/cecad1bde21cfc357cf70128144d6c83\"},\"headline\":\"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers\",\"datePublished\":\"2026-07-23T00:00:00+00:00\",\"dateModified\":\"2026-07-23T12:59:07+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/\"},\"wordCount\":2808,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/media.springernature.com\\\/m685\\\/springer-static\\\/image\\\/art%3A10.1038%2Fs41596-026-01411-4\\\/MediaObjects\\\/41596_2026_1411_Fig1_HTML.png\",\"keywords\":[\"activation\",\"Agents\",\"Contrast\",\"Formulation\",\"lipidshelled\",\"nanobubble\",\"ultrasound\"],\"articleSection\":[\"BioTechnology\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/\",\"url\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/\",\"name\":\"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers - Future News 24\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/media.springernature.com\\\/m685\\\/springer-static\\\/image\\\/art%3A10.1038%2Fs41596-026-01411-4\\\/MediaObjects\\\/41596_2026_1411_Fig1_HTML.png\",\"datePublished\":\"2026-07-23T00:00:00+00:00\",\"dateModified\":\"2026-07-23T12:59:07+00:00\",\"description\":\"Ultrasound is the second most common clinical imaging modality. Ultrasound image quality often suffers from poor contrast resolution and thus can greatly benefit from appropriate contrast agents. While micron-sized gas core particles (microbubbles) are clinically utilized in this space for applications such as echocardiography, a smaller agent could be more broadly applicable to enhance the sensitivity and specificity of disease detection. To this end, lipid-shelled nanobubbles have recently emerged as robust ultrasound contrast agents for both diagnostic and therapeutic purposes. They have been used in preclinical applications ranging from oncology to endocrinology and are notably relevant in diseases that involve pathological vasculature. Owing to their shell composition, nanobubbles are versatile and can be functionalized with fluorophores, targeting agents and therapeutic moieties. Here we provide the steps needed to formulate plain, fluorophore-conjugated, ligand-conjugated, hydrophilic dye-integrated and drug-loaded nanobubbles. The typical formulation for lipid-shelled nanobubbles takes place via self-assembly driven by mechanical agitation, followed by differential centrifugation. The process is deceptively simple, yet there are many nuances in the formulation process that must be followed to produce consistently successful nanobubble batches without contamination from microbubbles. Even minor deviation from the standard protocol can substantially affect nanobubble yield, stability, acoustic performance and batch-to-batch reproducibility. This procedure also details common pitfalls and their potential consequences for bubble quality and performance. The procedure requires 3 h to complete the formulation and activation of the plain nanobubbles by users with basic laboratory expertise. A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#primaryimage\",\"url\":\"https:\\\/\\\/media.springernature.com\\\/m685\\\/springer-static\\\/image\\\/art%3A10.1038%2Fs41596-026-01411-4\\\/MediaObjects\\\/41596_2026_1411_Fig1_HTML.png\",\"contentUrl\":\"https:\\\/\\\/media.springernature.com\\\/m685\\\/springer-static\\\/image\\\/art%3A10.1038%2Fs41596-026-01411-4\\\/MediaObjects\\\/41596_2026_1411_Fig1_HTML.png\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/2026\\\/07\\\/23\\\/s41596-026-01411-4\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/futurenews24.com\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#website\",\"url\":\"https:\\\/\\\/futurenews24.com\\\/\",\"name\":\"Future News 24\",\"description\":\"The Smart Hub for AI and Next-Gen Innovation\",\"publisher\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/futurenews24.com\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#organization\",\"name\":\"Future News 24\",\"url\":\"https:\\\/\\\/futurenews24.com\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/futurenews24.com\\\/wp-content\\\/uploads\\\/2026\\\/06\\\/fn24-favicon.png\",\"contentUrl\":\"https:\\\/\\\/futurenews24.com\\\/wp-content\\\/uploads\\\/2026\\\/06\\\/fn24-favicon.png\",\"width\":250,\"height\":250,\"caption\":\"Future News 24\"},\"image\":{\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#\\\/schema\\\/logo\\\/image\\\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/futurenews24.com\\\/#\\\/schema\\\/person\\\/cecad1bde21cfc357cf70128144d6c83\",\"name\":\"Future News 24\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/d57f07142d73cb5503ab2446ea7bc9ef3d0a5ba378d64a6157692311e42bf097?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/d57f07142d73cb5503ab2446ea7bc9ef3d0a5ba378d64a6157692311e42bf097?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/d57f07142d73cb5503ab2446ea7bc9ef3d0a5ba378d64a6157692311e42bf097?s=96&d=mm&r=g\",\"caption\":\"Future News 24\"},\"sameAs\":[\"https:\\\/\\\/futurenews24.com\"],\"url\":\"https:\\\/\\\/futurenews24.com\\\/index.php\\\/author\\\/mridulpahuja20\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers - Future News 24","description":"Ultrasound is the second most common clinical imaging modality. Ultrasound image quality often suffers from poor contrast resolution and thus can greatly benefit from appropriate contrast agents. While micron-sized gas core particles (microbubbles) are clinically utilized in this space for applications such as echocardiography, a smaller agent could be more broadly applicable to enhance the sensitivity and specificity of disease detection. To this end, lipid-shelled nanobubbles have recently emerged as robust ultrasound contrast agents for both diagnostic and therapeutic purposes. They have been used in preclinical applications ranging from oncology to endocrinology and are notably relevant in diseases that involve pathological vasculature. Owing to their shell composition, nanobubbles are versatile and can be functionalized with fluorophores, targeting agents and therapeutic moieties. Here we provide the steps needed to formulate plain, fluorophore-conjugated, ligand-conjugated, hydrophilic dye-integrated and drug-loaded nanobubbles. The typical formulation for lipid-shelled nanobubbles takes place via self-assembly driven by mechanical agitation, followed by differential centrifugation. The process is deceptively simple, yet there are many nuances in the formulation process that must be followed to produce consistently successful nanobubble batches without contamination from microbubbles. Even minor deviation from the standard protocol can substantially affect nanobubble yield, stability, acoustic performance and batch-to-batch reproducibility. This procedure also details common pitfalls and their potential consequences for bubble quality and performance. The procedure requires 3 h to complete the formulation and activation of the plain nanobubbles by users with basic laboratory expertise. A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/","og_locale":"en_US","og_type":"article","og_title":"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers - Future News 24","og_description":"Ultrasound is the second most common clinical imaging modality. Ultrasound image quality often suffers from poor contrast resolution and thus can greatly benefit from appropriate contrast agents. While micron-sized gas core particles (microbubbles) are clinically utilized in this space for applications such as echocardiography, a smaller agent could be more broadly applicable to enhance the sensitivity and specificity of disease detection. To this end, lipid-shelled nanobubbles have recently emerged as robust ultrasound contrast agents for both diagnostic and therapeutic purposes. They have been used in preclinical applications ranging from oncology to endocrinology and are notably relevant in diseases that involve pathological vasculature. Owing to their shell composition, nanobubbles are versatile and can be functionalized with fluorophores, targeting agents and therapeutic moieties. Here we provide the steps needed to formulate plain, fluorophore-conjugated, ligand-conjugated, hydrophilic dye-integrated and drug-loaded nanobubbles. The typical formulation for lipid-shelled nanobubbles takes place via self-assembly driven by mechanical agitation, followed by differential centrifugation. The process is deceptively simple, yet there are many nuances in the formulation process that must be followed to produce consistently successful nanobubble batches without contamination from microbubbles. Even minor deviation from the standard protocol can substantially affect nanobubble yield, stability, acoustic performance and batch-to-batch reproducibility. This procedure also details common pitfalls and their potential consequences for bubble quality and performance. The procedure requires 3 h to complete the formulation and activation of the plain nanobubbles by users with basic laboratory expertise. A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.","og_url":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/","og_site_name":"Future News 24","article_published_time":"2026-07-23T00:00:00+00:00","article_modified_time":"2026-07-23T12:59:07+00:00","og_image":[{"url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png","type":"","width":"","height":""}],"author":"Future News 24","twitter_card":"summary_large_image","twitter_image":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png","twitter_misc":{"Written by":"Future News 24","Est. reading time":"14 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#article","isPartOf":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/"},"author":{"name":"Future News 24","@id":"https:\/\/futurenews24.com\/#\/schema\/person\/cecad1bde21cfc357cf70128144d6c83"},"headline":"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers","datePublished":"2026-07-23T00:00:00+00:00","dateModified":"2026-07-23T12:59:07+00:00","mainEntityOfPage":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/"},"wordCount":2808,"commentCount":0,"publisher":{"@id":"https:\/\/futurenews24.com\/#organization"},"image":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#primaryimage"},"thumbnailUrl":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png","keywords":["activation","Agents","Contrast","Formulation","lipidshelled","nanobubble","ultrasound"],"articleSection":["BioTechnology"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/","url":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/","name":"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers - Future News 24","isPartOf":{"@id":"https:\/\/futurenews24.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#primaryimage"},"image":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#primaryimage"},"thumbnailUrl":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png","datePublished":"2026-07-23T00:00:00+00:00","dateModified":"2026-07-23T12:59:07+00:00","description":"Ultrasound is the second most common clinical imaging modality. Ultrasound image quality often suffers from poor contrast resolution and thus can greatly benefit from appropriate contrast agents. While micron-sized gas core particles (microbubbles) are clinically utilized in this space for applications such as echocardiography, a smaller agent could be more broadly applicable to enhance the sensitivity and specificity of disease detection. To this end, lipid-shelled nanobubbles have recently emerged as robust ultrasound contrast agents for both diagnostic and therapeutic purposes. They have been used in preclinical applications ranging from oncology to endocrinology and are notably relevant in diseases that involve pathological vasculature. Owing to their shell composition, nanobubbles are versatile and can be functionalized with fluorophores, targeting agents and therapeutic moieties. Here we provide the steps needed to formulate plain, fluorophore-conjugated, ligand-conjugated, hydrophilic dye-integrated and drug-loaded nanobubbles. The typical formulation for lipid-shelled nanobubbles takes place via self-assembly driven by mechanical agitation, followed by differential centrifugation. The process is deceptively simple, yet there are many nuances in the formulation process that must be followed to produce consistently successful nanobubble batches without contamination from microbubbles. Even minor deviation from the standard protocol can substantially affect nanobubble yield, stability, acoustic performance and batch-to-batch reproducibility. This procedure also details common pitfalls and their potential consequences for bubble quality and performance. The procedure requires 3 h to complete the formulation and activation of the plain nanobubbles by users with basic laboratory expertise. A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical space.","breadcrumb":{"@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#primaryimage","url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png","contentUrl":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41596-026-01411-4\/MediaObjects\/41596_2026_1411_Fig1_HTML.png"},{"@type":"BreadcrumbList","@id":"https:\/\/futurenews24.com\/index.php\/2026\/07\/23\/s41596-026-01411-4\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/futurenews24.com\/"},{"@type":"ListItem","position":2,"name":"Formulation and activation of lipid-shelled nanobubble ultrasound distinction brokers"}]},{"@type":"WebSite","@id":"https:\/\/futurenews24.com\/#website","url":"https:\/\/futurenews24.com\/","name":"Future News 24","description":"The Smart Hub for AI and Next-Gen Innovation","publisher":{"@id":"https:\/\/futurenews24.com\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/futurenews24.com\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/futurenews24.com\/#organization","name":"Future News 24","url":"https:\/\/futurenews24.com\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/futurenews24.com\/#\/schema\/logo\/image\/","url":"https:\/\/futurenews24.com\/wp-content\/uploads\/2026\/06\/fn24-favicon.png","contentUrl":"https:\/\/futurenews24.com\/wp-content\/uploads\/2026\/06\/fn24-favicon.png","width":250,"height":250,"caption":"Future News 24"},"image":{"@id":"https:\/\/futurenews24.com\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/futurenews24.com\/#\/schema\/person\/cecad1bde21cfc357cf70128144d6c83","name":"Future News 24","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/d57f07142d73cb5503ab2446ea7bc9ef3d0a5ba378d64a6157692311e42bf097?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/d57f07142d73cb5503ab2446ea7bc9ef3d0a5ba378d64a6157692311e42bf097?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/d57f07142d73cb5503ab2446ea7bc9ef3d0a5ba378d64a6157692311e42bf097?s=96&d=mm&r=g","caption":"Future News 24"},"sameAs":["https:\/\/futurenews24.com"],"url":"https:\/\/futurenews24.com\/index.php\/author\/mridulpahuja20\/"}]}},"_links":{"self":[{"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/posts\/2738","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/comments?post=2738"}],"version-history":[{"count":1,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/posts\/2738\/revisions"}],"predecessor-version":[{"id":2739,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/posts\/2738\/revisions\/2739"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/media\/2740"}],"wp:attachment":[{"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/media?parent=2738"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/categories?post=2738"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/futurenews24.com\/index.php\/wp-json\/wp\/v2\/tags?post=2738"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}