{"id":1059,"date":"2026-06-16T00:00:00","date_gmt":"2026-06-16T00:00:00","guid":{"rendered":"https:\/\/futurenews24.com\/index.php\/2026\/06\/16\/s41428-026-01212-2\/"},"modified":"2026-06-16T04:59:38","modified_gmt":"2026-06-16T04:59:38","slug":"s41428-026-01212-2","status":"publish","type":"post","link":"https:\/\/futurenews24.com\/index.php\/2026\/06\/16\/s41428-026-01212-2\/","title":{"rendered":"pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein supply"},"content":{"rendered":"<p><br \/>\n<\/p>\n<div id=\"\">\n<p class=\"c-article-references__text\" id=\"ref-CR1\">S\u00e1nchez-Navarro M. Advances in peptide-mediated cytosolic supply of proteins. Adv Drug Deliv Rev. 2021;171:187\u201398.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR2\">Lagass\u00e9 HAD, Alexaki A, Simhadri VL, Katagiri NH, Jankowski W, Sauna ZE, et al. Latest advances in therapeutic protein drug growth. F1000Research. 2017;6:113.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR3\">Urquhart L. High product forecasts for 2020. Nat Rev Drug Discov. 2020;19:2.<\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR4\">Zhang Y, R\u00f8ise JJ, Lee Okay, Li J, Murthy N. Latest developments in intracellular protein supply. Curr Opin Biotechnol. 2018;52:25\u201331.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR5\">Son H, Shin J, Park J. Latest progress in nanomedicine-mediated cytosolic supply. RSC Adv. 2023;13:9788\u201399.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR6\">El-Sayed A, Futaki S, Harashima H. Supply of macromolecules utilizing arginine-rich cell-penetrating peptides: methods to beat endosomal entrapment. AAPS J. 2009;11:13\u201322.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR7\">He W, Xing X, Wang X, Wu D, Wu W, Guo J, et al. Nanocarrier-mediated cytosolic supply of biopharmaceuticals. Adv Funct Mater. 2020;30:1910566.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR8\">Kwon YJ. Polymeric vectors for gene and protein supply in vitro and in vivo. Acc Chem Res. 2012;45:1077\u201387.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR9\">Niikura Okay, Horisawa Okay, Doi N. Fusogenic peptide from sea urchin promotes intracellular supply by way of endosomal escape. J Management Launch. 2015;212:85\u201393.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR10\">Erazo-Oliveras A, Najjar Okay, Dayani L, Wang TY, Johnson GA, Pellois JP. Protein supply into stay cells by incubation with an endosomolytic agent. Nat Strategies. 2014;11:861\u20137.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR11\">Parente RA, Nadasdi L, Subbarao NK, Szoka FC Jr. Affiliation of a pH-sensitive peptide with membrane vesicles: function of amino acid sequence. Biochemistry. 1990;29:8713\u20139.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR12\">Li W, Nicol F, Szoka FC Jr. GALA: an artificial pH-responsive amphipathic peptide for drug and gene supply. Adv Drug Deliv Rev. 2004;56:967\u201385.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR13\">Akishiba M, Takeuchi T, Kawaguchi Y, Sakamoto Okay, Yu HH, Nakase I, et al. Cytosolic antibody supply by lipid-sensitive endosomolytic peptide. Nat Chem. 2017;9:751\u201361.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR14\">Azuma Y, Imai H, Kawaguchi Y, Nakase I, Kimura H, Futaki S. Modular redesign of cationic lytic peptide for endosomal escape. Angew Chem Int Ed. 2018;57:12771\u20134.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR15\">Kobayashi S, Nakase I, Kawabata N, Yu HH, Pujals S, Imanishi M, et al. Cytosolic focusing on utilizing a pH-dependent fusogenic peptide with cationic liposomes. Bioconjug Chem. 2009;20:953\u20139.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR16\">Akishiba M, Futaki S. Inducible membrane permeabilization by attenuated lytic peptides. Mol Pharm. 2019;16:2540\u20138.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR17\">Nguyen MA, Wyatt H, Susser L, Geoffrion M, Rasheed A, Duchez AC, et al. Proton sponge-mediated endosomal escape and intracellular drug supply. ACS Nano. 2019;13:6491\u2013505.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR18\">Kowalski PS, Capasso Palmiero U, Huang Y, Rudra A, Langer R, Anderson DG. Nanoparticle-mediated intracellular protein supply utilizing proton sponge impact. Adv Mater. 2018;30:1801151.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR19\">Zhou J, Liu J, Cheng CJ, Patel TR, Weller CE, Piepmeier JM, et al. Nanomaterial-based methods to beat the endosomal barrier. Nat Mater. 2012;11:82\u201390.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR20\">Qiu N, Gao J, Liu Q, Wang J, Shen Y. Biomacromolecular supply by endosomal escape methods. Biomacromolecules. 2018;19:2308\u201318.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR21\">Pack DW, Putnam D, Langer R. Design and growth of endosome-disruptive gene supply programs. Biotechnol Bioeng. 2000;67:217\u201323.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR22\">Yang Y, Xu Z, Jiang J, Gao Y, Gu W, Chen L, et al. pH-responsive supply programs for cytosolic protein launch. J Management Launch. 2008;127:273\u201380.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR23\">Ahmad A, Khan JM, Haque S. Photochemical endosomal escape: latest insights and challenges. Biochimie. 2019;160:61\u201372.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR24\">Brock DJ, Kondow-McConaghy HM, Hager EC, Pellois J-P. Endosomal escape of delivered proteins facilitated by photochemical internalization. Bioconjug Chem. 2019;30:293\u2013303.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR25\">Ohtsuki T, Miki S, Kobayashi S, Haraguchi T, Nakata E, Hirakawa Okay, et al. Mild-induced endosomal escape and lysosomal evasion with a pH-activatable photosensitizer. Sci Rep. 2016;5:18577.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR26\">Mellman I, Fuchs R, Helenius A. Acidification of the endocytic and exocytic pathways. Annu Rev Biochem. 1986;55:663\u2013700.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR27\">Minami T, Matsumoto S, Sanada Y, Waku T, Tanaka N, Sakurai Okay. Rod-like structure and cross-sectional construction of an amyloid protofilament-like peptide supermolecule in aqueous answer. Polym J. 2016;48:197\u2013202.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR28\">Waku T, Nishigaki S, Kitagawa Y, Koeda S, Kawabata Okay, Kunugi S, et al. Impact of the hydrophilic-hydrophobic stability of antigen-loaded peptide nanofibers on their mobile uptake, mobile toxicity, and immune stimulatory properties. Int J Mol Sci. 2019;20:3781.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR29\">Waku T, Kasai A, Kobori A, Tanaka N. Investigation on the interactions between self-assembled \u03b2-sheet peptide nanofibers and mannequin cell membranes. Int J Mol Sci. 2020;21:9518.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR30\">Semisotnov GV, Rodionova NV, Razgulyaev OI, Uversky VN, Gripas AF, Gilmanshin RI. Examine of the molten globule intermediate state by hydrophobic fluorescent probe. Biopolymers. 1991;31:119\u201328.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR31\">Yamaguchi S, Mannen T, Nagamune T. Analysis of floor hydrophobicity of immobilized protein with a floor plasmon resonance sensor. Biotechnol Lett. 2004;26:1081\u20136.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR32\">Cardamone M, Puri N. Spectrofluorimetric evaluation of the floor hydrophobicity of proteins. Biochem J. 1992;282:589\u201393.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR33\">Akagi T, Kim H, Akashi M. pH-dependent disruption of erythrocyte membrane by amphiphilic poly(amino acid) nanoparticles. J Biomater Sci Polym Ed. 2010;21:315\u201328.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR34\">Plank C, Oberhauser B, Mechtler Okay, Koch C, Wagner E. Impact of endosome-disruptive peptides on gene switch utilizing artificial virus-like programs. J Biol Chem. 1994;269:12918\u201324.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR35\">Martin MM, Lindqvist L. The pH dependence of fluorescein fluorescence. J Lumin. 1975;10:381\u201390.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR36\">Toyoda M, Miura Y, Kobayashi M, Tsuda M, Nomoto T, Honda Y, et al. Synthesis and optimization of ethylenediamine-based zwitterion on polymer aspect chain for recognizing slender tumorous pH home windows. Biomacromolecules. 2024;25:7788\u201398.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<br \/>\n    PubMed Central\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR37\">Yuba E, Kono Y, Harada A, Yokoyama S, Arai M, Kubo Okay, et al. The appliance of pH-sensitive polymer-lipids to antigen supply for most cancers immunotherapy. Biomaterials. 2013;34:5711\u201321.<\/p>\n<p class=\"c-article-references__links u-hide-print\">Article\u00a0<br \/>\n    CAS\u00a0<br \/>\n    PubMed\u00a0<\/p>\n<p>                    Google Scholar\u00a0\n                <\/p>\n<\/div>\n<p><br \/>\n<br \/><a href=\"https:\/\/www.nature.com\/articles\/s41428-026-01212-2\">Source link <\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>S\u00e1nchez-Navarro M. Advances in peptide-mediated cytosolic supply of proteins. Adv Drug Deliv Rev. 2021;171:187\u201398. Article\u00a0 PubMed\u00a0 Google Scholar\u00a0 Lagass\u00e9 HAD, Alexaki A, Simhadri VL, Katagiri NH, Jankowski W, Sauna ZE, et al. Latest advances in therapeutic protein drug growth. F1000Research. 2017;6:113. Article\u00a0 PubMed\u00a0 PubMed Central\u00a0 Google Scholar\u00a0 Urquhart L. High product forecasts for 2020. Nat [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1061,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"fifu_image_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41428-026-01212-2\/MediaObjects\/41428_2026_1212_Figa_HTML.png","fifu_image_alt":"","jnews-multi-image_gallery":[],"jnews_single_post":[],"jnews_primary_category":[],"jnews_override_bookmark_settings":[],"jnews_social_meta":[],"jnews_override_counter":[],"footnotes":""},"categories":[10],"tags":[1434,1439,1441,1438,597,1437,1436,1435,1433,1440],"class_list":["post-1059","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biotechnology","tag-amphiphilic","tag-cytosolic","tag-delivery","tag-endosomal","tag-escape","tag-facilitate","tag-nanofibers","tag-peptide","tag-phresponsive","tag-protein"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein supply - Future News 24<\/title>\n<meta name=\"description\" content=\"The therapeutic use of proteins often depends on cytosolic access, which is frequently limited by endosomal trapping. Here, we developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. We prepared a series of NFs by varying the number of glutamic acid residues (CE1, CE2, and CE3) appended to &#946;-sheet-forming peptides. The resulting nanofibers were uniform in width and presented hydrophobic surface domains. Among these, CE2 NFs emerged as optimal: red blood cell hemolysis assays showed strong activity at mildly acidic pH, but they remained inert at neutral pH. In cells, a pH-sensitive FITC&#8211;PEG probe revealed a time-dependent increase in fluorescence with CE2 NFs, which is consistent with the translocation of the probe from acidic endosomes to the neutral cytosol, supporting endosomal-membrane destabilization by CE2 NFs. Furthermore, in OVA-treated dendritic cells, CE2 NFs increased surface SIINFEKL&#8211;H-2Kb presentation to levels comparable to the L17E peptide, a reagent known to promote cytosolic protein delivery. These results suggest that CE2 NFs facilitate the cytosolic translocation of coadministered proteins. In contrast, monomeric peptides showed no endosomal escape-promoting activity, supporting the necessity of self-assembled nanofiber architecture. Collectively, these findings suggest that pH-responsive peptide nanofibers serve as effective facilitators of cytosolic protein delivery via endosome-selective membrane destabilization. We developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. The NFs were based on &#946;-sheet-forming peptides conjugated with oligoglutamic acid segments, and the number of Glu residues was varied to optimize pH-responsive activity. Optimized NFs showed strong membrane-destabilizing activity at mildly acidic pH but remained inert at neutral pH. When coadministered with ovalbumin (OVA) to dendritic cells, these NFs increased major histocompatibility complex (MHC) class I presentation of the OVA-derived SIINFEKL epitope, supporting cytosolic delivery of OVA.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/futurenews24.com\/index.php\/2026\/06\/16\/s41428-026-01212-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein supply - Future News 24\" \/>\n<meta property=\"og:description\" content=\"The therapeutic use of proteins often depends on cytosolic access, which is frequently limited by endosomal trapping. Here, we developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. We prepared a series of NFs by varying the number of glutamic acid residues (CE1, CE2, and CE3) appended to &#946;-sheet-forming peptides. The resulting nanofibers were uniform in width and presented hydrophobic surface domains. Among these, CE2 NFs emerged as optimal: red blood cell hemolysis assays showed strong activity at mildly acidic pH, but they remained inert at neutral pH. In cells, a pH-sensitive FITC&#8211;PEG probe revealed a time-dependent increase in fluorescence with CE2 NFs, which is consistent with the translocation of the probe from acidic endosomes to the neutral cytosol, supporting endosomal-membrane destabilization by CE2 NFs. Furthermore, in OVA-treated dendritic cells, CE2 NFs increased surface SIINFEKL&#8211;H-2Kb presentation to levels comparable to the L17E peptide, a reagent known to promote cytosolic protein delivery. These results suggest that CE2 NFs facilitate the cytosolic translocation of coadministered proteins. In contrast, monomeric peptides showed no endosomal escape-promoting activity, supporting the necessity of self-assembled nanofiber architecture. Collectively, these findings suggest that pH-responsive peptide nanofibers serve as effective facilitators of cytosolic protein delivery via endosome-selective membrane destabilization. We developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. The NFs were based on &#946;-sheet-forming peptides conjugated with oligoglutamic acid segments, and the number of Glu residues was varied to optimize pH-responsive activity. Optimized NFs showed strong membrane-destabilizing activity at mildly acidic pH but remained inert at neutral pH. 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