{"version":"1.0","provider_name":"Future News 24","provider_url":"https:\/\/futurenews24.com","author_name":"Future News 24","author_url":"https:\/\/futurenews24.com\/index.php\/author\/mridulpahuja20\/","title":"Institution of an in vitro tradition and regeneration protocol for the native Chilean grass Polypogon australis Brong - Future News 24","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"2gGYcngFsz\"><a href=\"https:\/\/futurenews24.com\/index.php\/2026\/09\/04\/s41598-026-61154-w\/\">Institution of an in vitro tradition and regeneration protocol for the native Chilean grass Polypogon australis Brong<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/futurenews24.com\/index.php\/2026\/09\/04\/s41598-026-61154-w\/embed\/#?secret=2gGYcngFsz\" width=\"600\" height=\"338\" title=\"&#8220;Institution of an in vitro tradition and regeneration protocol for the native Chilean grass Polypogon australis Brong&#8221; &#8212; Future News 24\" data-secret=\"2gGYcngFsz\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/futurenews24.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","thumbnail_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41598-026-61154-w\/MediaObjects\/41598_2026_61154_Fig1_HTML.jpg","thumbnail_width":1024,"thumbnail_height":1024,"description":"Polypogon australis Brong. is a native Chilean grass frequently found colonizing metal-rich mine tailings, yet it lacks an established in vitro regeneration system to support controlled physiological and biotechnological studies. Here, we report a reproducible protocol for seed germination, callus induction, and plant regeneration using coleoptile&#8211;mesocotyl explants. Surface-sterilized seeds were germinated on Murashige and Skoog (MS) medium supplemented with sucrose, achieving a cumulative germination percentage of 47.67&#8201;&#177;&#8201;3.15% after 15 days. The coleoptile&#8211;mesocotyl explant proved highly responsive to culture on callus induction medium (CIM) supplemented with dicamba, resulting in a callus induction frequency of 30.55&#8201;&#177;&#8201;11.96% after 3&#8211;5 weeks. Induced calli were predominantly embryogenic, with embryogenic calli representing 65.42&#8201;&#177;&#8201;8.61% of the total callus population. Embryogenic calli regenerated complete plantlets with a regeneration efficiency of 45.0&#8201;&#177;&#8201;23.3%. Organogenic structures, including primary shoots and roots, developed directly from embryogenic calli maintained on callus induction medium (CIM) supplemented with dicamba, without transfer to a specialized regeneration medium containing organogenesis-promoting growth regulators. After the initiation of organogenesis, cultures were exposed to a 16&amp;nbsp;h light\/8&amp;nbsp;h dark photoperiod while remaining on CIM, and regenerated plantlets were subsequently transferred to MS+10&amp;nbsp;S medium for further growth and elongation. This study establishes the first complete in vitro regeneration system for P. australis, providing a practical framework for future physiological studies, large-scale propagation, genetic transformation, and genome engineering applications in this ecologically relevant native Chilean grass."}