{"version":"1.0","provider_name":"Future News 24","provider_url":"https:\/\/futurenews24.com","author_name":"Future News 24","author_url":"https:\/\/futurenews24.com\/index.php\/author\/mridulpahuja20\/","title":"Synthesis and molecular docking of novel pyrazole, pyrimidine, and pyridine derivatives as potent antimicrobial, antibiofilm, and anticancer brokers - Future News 24","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"IsxZ5SekPE\"><a href=\"https:\/\/futurenews24.com\/index.php\/2026\/08\/08\/s41598-026-65144-w\/\">Synthesis and molecular docking of novel pyrazole, pyrimidine, and pyridine derivatives as potent antimicrobial, antibiofilm, and anticancer brokers<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/futurenews24.com\/index.php\/2026\/08\/08\/s41598-026-65144-w\/embed\/#?secret=IsxZ5SekPE\" width=\"600\" height=\"338\" title=\"&#8220;Synthesis and molecular docking of novel pyrazole, pyrimidine, and pyridine derivatives as potent antimicrobial, antibiofilm, and anticancer brokers&#8221; &#8212; Future News 24\" data-secret=\"IsxZ5SekPE\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/futurenews24.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","thumbnail_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41598-026-65144-w\/MediaObjects\/41598_2026_65144_Fig1_HTML.png","thumbnail_width":1024,"thumbnail_height":1024,"description":"The development of novel antimicrobial and anticancer agents remains a priority due to rising drug resistance and high systemic toxicity of current treatments. A series of novel pyrazole (2&#8211;6), pyrimidine (7&#8211;10), and pyridine\/oxazinone (11&#8211;14) derivatives were synthesized from a chalcone scaffold (1). They were evaluated for antimicrobial, antibiofilm (Pseudomonas aeruginosa), and cytotoxic (HepG2 cells) activities. Molecular docking and qRT-PCR were performed to study their mechanism. Pyrazoles 3&#8211;5 and oxazinone 13 showed potent antibacterial activity against S. aureus (MIC&#8201;=&#8201;2&#8211;3.12&amp;nbsp;&#181;g\/mL). Compounds 4 and 13 effectively eradicated P. aeruginosa biofilms, achieving a&#8201;&#8805;&#8201;5 log10 reduction within 4&amp;nbsp;h at 0.8&#8201;&#215;&#8201;MIC, driven by disruption of the bacterial respiratory chain. For anticancer activity, compounds 10 and 13 selectively reduced HepG2 cell viability to 35&#8211;40% via oxidative stress-mediated apoptosis. Mechanistically, compound 4 reduced gyrB expression in E. coli by approximately 4.6-fold, while molecular docking supported its interaction with the ATP-binding pocket of DNA gyrase. Overall, the functionalized heterocycles, particularly compounds 4 and 13, represent promising dual-acting candidates with potent antibacterial, antibiofilm, and selective anticancer activities."}