{"version":"1.0","provider_name":"Future News 24","provider_url":"https:\/\/futurenews24.com","author_name":"Future News 24","author_url":"https:\/\/futurenews24.com\/index.php\/author\/mridulpahuja20\/","title":"Piperazine linked chitosan schiff base nanoparticles as a novel antibiofilm and antibacterial technique towards clinically related pathogens - Future News 24","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"k9hl95rqhy\"><a href=\"https:\/\/futurenews24.com\/index.php\/2026\/08\/06\/s41598-026-63745-z\/\">Piperazine linked chitosan schiff base nanoparticles as a novel antibiofilm and antibacterial technique towards clinically related pathogens<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/futurenews24.com\/index.php\/2026\/08\/06\/s41598-026-63745-z\/embed\/#?secret=k9hl95rqhy\" width=\"600\" height=\"338\" title=\"&#8220;Piperazine linked chitosan schiff base nanoparticles as a novel antibiofilm and antibacterial technique towards clinically related pathogens&#8221; &#8212; Future News 24\" data-secret=\"k9hl95rqhy\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/futurenews24.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","thumbnail_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41598-026-63745-z\/MediaObjects\/41598_2026_63745_Fig1_HTML.png","thumbnail_width":1024,"thumbnail_height":1024,"description":"The development of multifunctional antimicrobial materials capable of targeting both planktonic bacteria and biofilm-associated infections remains a critical challenge in combating antimicrobial resistance. In this study, a novel piperazine-linked chitosan Schiff base (Cs-TPA-PiP) and its ionically crosslinked nanoparticle formulation (Cs-TPA-PiP NPs) were synthesized and structurally characterized. The antimicrobial potential of both Cs-TPA-PiP and Cs-TPA-PiP NPs was evaluated against a panel of nine standard clinically significant bacterial strains. The compounds demonstrated significant and broad-spectrum antibacterial activity. The minimum inhibitory concentration (MIC) values demonstrated potent efficacy, with Cs-TPA-PiP and its Cs-TPA-PiP NPs ranging from 0.63 to 2.50&amp;nbsp;mg\/mL and 1.00&#8211;5.00&amp;nbsp;mg\/mL, respectively. Notably, both agents exhibited a strong dose-dependent inhibitory effect on biofilm formation. While Cs-TPA-PiP showed lower MIC values against planktonic cells, the corresponding Cs-TPA-PiP NPs with an ultra-small spherical size of 15.6&amp;nbsp;nm exhibited superior antibiofilm performance, ranging from 73.00% to 95.00% inhibition of biofilm biomass at 1&#215; MIC in strong biofilm-producing strains. Transmission electron microscopy (TEM) confirmed severe morphological alterations and membrane disruption in treated bacterial cells, consistent with a membrane-targeting mechanism. In silico molecular docking studies suggested that the compound has favorable binding affinity for the critical bacterial cell wall target, Sortase A, thereby identifying it as a potential theoretical target requiring further validation. Our findings collectively establish Cs-TPA-PiP and its Cs-TPA-PiP NPs as effective antibacterial and anti-biofilm candidates, with their activity primarily attributed to membrane disruption. The proposed role of Sortase A inhibition remains hypothetical and warrants further investigation. These findings highlight their potential as multifunctional antibacterial platforms for managing biofilm-associated and resistant bacterial infections."}