{"version":"1.0","provider_name":"Future News 24","provider_url":"https:\/\/futurenews24.com","author_name":"Future News 24","author_url":"https:\/\/futurenews24.com\/index.php\/author\/mridulpahuja20\/","title":"pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein supply - Future News 24","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"iX9VPUPPr3\"><a href=\"https:\/\/futurenews24.com\/index.php\/2026\/06\/16\/s41428-026-01212-2\/\">pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein supply<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/futurenews24.com\/index.php\/2026\/06\/16\/s41428-026-01212-2\/embed\/#?secret=iX9VPUPPr3\" width=\"600\" height=\"338\" title=\"&#8220;pH-responsive amphiphilic peptide nanofibers facilitate endosomal escape for cytosolic protein supply&#8221; &#8212; Future News 24\" data-secret=\"iX9VPUPPr3\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/futurenews24.com\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","thumbnail_url":"https:\/\/media.springernature.com\/m685\/springer-static\/image\/art%3A10.1038%2Fs41428-026-01212-2\/MediaObjects\/41428_2026_1212_Figa_HTML.png","thumbnail_width":1024,"thumbnail_height":1024,"description":"The therapeutic use of proteins often depends on cytosolic access, which is frequently limited by endosomal trapping. Here, we developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. We prepared a series of NFs by varying the number of glutamic acid residues (CE1, CE2, and CE3) appended to &#946;-sheet-forming peptides. The resulting nanofibers were uniform in width and presented hydrophobic surface domains. Among these, CE2 NFs emerged as optimal: red blood cell hemolysis assays showed strong activity at mildly acidic pH, but they remained inert at neutral pH. In cells, a pH-sensitive FITC&#8211;PEG probe revealed a time-dependent increase in fluorescence with CE2 NFs, which is consistent with the translocation of the probe from acidic endosomes to the neutral cytosol, supporting endosomal-membrane destabilization by CE2 NFs. Furthermore, in OVA-treated dendritic cells, CE2 NFs increased surface SIINFEKL&#8211;H-2Kb presentation to levels comparable to the L17E peptide, a reagent known to promote cytosolic protein delivery. These results suggest that CE2 NFs facilitate the cytosolic translocation of coadministered proteins. In contrast, monomeric peptides showed no endosomal escape-promoting activity, supporting the necessity of self-assembled nanofiber architecture. Collectively, these findings suggest that pH-responsive peptide nanofibers serve as effective facilitators of cytosolic protein delivery via endosome-selective membrane destabilization. We developed pH-responsive amphiphilic peptide nanofibers (NFs) that may facilitate cytosolic protein delivery by selectively destabilizing endosomal membranes. The NFs were based on &#946;-sheet-forming peptides conjugated with oligoglutamic acid segments, and the number of Glu residues was varied to optimize pH-responsive activity. Optimized NFs showed strong membrane-destabilizing activity at mildly acidic pH but remained inert at neutral pH. When coadministered with ovalbumin (OVA) to dendritic cells, these NFs increased major histocompatibility complex (MHC) class I presentation of the OVA-derived SIINFEKL epitope, supporting cytosolic delivery of OVA."}